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Idealmente, todos los buffers usados en SEC y en todos los tipos de cromatografa deben de ser filtrados con una membrana de 0.2 jum y desgasificados (Cutler, 2008)
Metabolically active forms of B12 such as methylcobalamin and adenocobalamin require no further conversion in the body

The patients history of optic neuritis, characterized by acute unilateral vision loss, severe eye pain with movement, and field defects documented on visual field testing, is a notable clinical feature
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