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10.1073/pnas.1209965109 125 NepogodievD.MartinJ.BiccardB.MakupeA.BhanguA.National Institute for Health Research Global Health Research Unit on Global Surgery (2019)

Differentiation of LS patient derived iPSCs into neural lineage in both 2D neural cultures and 3D organoids revealed a consistent trend across all LS cerebral organoids, which exhibited impaired growth at various time intervals

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Western blot Total protein extracts from macrophages (1 10 6 cells) were prepared using RIPA lysis buffer containing protease and phosphatase inhibitors (Thermo Fisher Scientific)