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Subsequently, cells were dissociated into single-cell suspensions by treatment with Trypsin-EDTA (Gibco), followed by several washing steps

Often, this means storing them in their original containers in a cool, dry place out of direct sunlight

In the future, it will be necessary to combine gene editing, cell engineering, and materials science to develop new technologies for precise temporal and spatial regulation of mitochondrial function, while also promoting clinical trials to verify their safety and efficacy

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