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doi: 10.3390/ijms21249639 301 FongLKYangMMDos Santos ChavesRReynaSMLangnessVFWoodruffGet al

The bacterial pellet was then resuspended in 800 L of GMML liquid medium (M9 salts, 1% glycerol, 1 mM MgSO 4 and 0.1 mM CaCl 2 and 0.3 mM Leucine) containing Amp, Spe, IPTG (0.5 mM) and a specific ncAA (1 mM), This suspension was then transferred to 96-deep-well plates (2.2 mL) and incubated at 37 C, 220 rpm for 11 h, with monitoring of fluorescence intensity (excitation 485 nm and emission 515 nm) as well as OD 600 by Microplate Readers

Yan-Qing W, Hong-Mei Z (2012) Spectral studies on the interaction between Cu2+ and urease