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Normalization counts of reads were done by mapping reads per kilobase of transcript per million (RPKM) to annotated Ensembl genes using the open-source software Bioconductor easyRNASeq v1.6.0 package on R version 3.0 (Lucent Technologies, Inc., Murray Hill, NJ)

Similarly, the number of infiltrating neutrophils, monocytes, and eosinophils that increased in both groups of mice during acute CDI, remained significantly elevated in HFD-fed mice till day 10 after infection (Fig
