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A decrease in the level of this natural antioxidant is one of the earliest biochemical changes seen in patients with the disease and is associated with oxidative stress and neurodegeneration
Material and methods Genomic DNA samples were extracted from 297 healthy unrelated individuals by a ReliaPrep Blood gDNA Miniprep kit (Promega, USA), and genotyped for allelic variation in GSTM1 and GSTT1 genotypes by multiplex polymerase chain reaction
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