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An increased risk of retinopathy resulted in updated ophthalmology guidelines that recommended a maximal daily dose of 5.0 mg/kg actual body weight for hydroxychloroquine
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Then, the media was removed, washed with 1X PBS, and stained with a 1:1 mixture of AO and EtBr (100 g/mL each) for 30 min
During this stage, various receptors such as TLRs, NOD-like receptors, and TNF receptors are activated to send signals that alert the immune system to infection or injury [30, 31]
