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Similarly, if your protein has no cysteines then you can mutate a specific residue of your choosing to cysteine, and that will be the only site for maleimide biotinylation
doi: 10.3390/ijms21030950 33
Measurement is limited primarily to the underestimation of GSH and overestimation GSSG as a result of auto-oxidation of GSH
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Thats exactly why so many in-vitro experiments use fibroblast cultures as their starting point when studying this compound
