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Trypsin without the proline restriction enzyme option was used, with two allowed miscleavages

TNFalpha-induced MMP-9 promotes macrophage recruitment into injured peripheral nerve

The plate was incubated at room temperature for 510 min, before luminescence was measured using a Victor 3 1420 Multilabel Counter (Wallac 1420 Workstation, version 3.00 revision 5
