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Samples were resolved by SDS-PAGE, transferred to nitrocellulose membranes and analyzed for protein AMPylation using 0.65 g/ml -AMPylation (clone 1G11), -SelO (Abcam EPR11968, 1:1000), -GAPDH (ThermoFisher MA5-15738, 1:20000), -Glud1 (proteinTech 14299, 1:5000), -pdhB (proteinTech 14744, 1:3000), -acat1 (proteinTech 16215, 1:1000), or -OGDH (proteinTech 66285, 1:3000)

In kidney transplantation, ischemia time and donor factors dictate initial graft function