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Allow to rest for 10 minutes Make serial dilutions of the standard according to the following table: Dilute anti-rabbit IgG HRP for both Human Tau (Total) and Human pTau (Ser199) by allowing it to reach room temperature, then use the following table Dilute wash buffer from 25X concentrate: Amount of wash buffer = (x)*(8)*(400)*(4)*(3), where x = number of strips of ELISA plate Dilute 24 volumes with deionizer water (ie 5ml concentrate into 120ml water) Warm well strips and determine how many are needed, insert them into the frame Load standards: Load 100 L of standard diluent buffer into ZERO wells Load 100 L of each standard concentrations (both total Tau and pTau) to wells in DUPLICATE Load samples: To each well that will be analyzed for total Tau, add 85l standard diluent buffer + 15 L cell lysate To each well that will be analyzed for pTau (Ser199) add 50 L standard diluent buffer + 50 L cell lysate BE SURE TO NOTE WHICH WELLS/PLATES WILL BE ANALYZED for total Tau versus pTau

But grape-seed extract does contain concentrated doses of other phytonutrients that are harder to attain from the diet.* Grape-seed extract should be looked at for what it isa supplement to a healthy diet and not a replacement for a healthy diet