interaction curcumin glutathione b. Hypothetical Michael adduct of gluthathione and curcumin, pictured The Credible Role of Curcumin

It is natural to speculate whether the inhibition of GS and GCL is an important evolutionary mechanism to keep GSH high, but not too high, in females during the childbearing years

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The GST-fusion protein first binds to glutathione agarose, and then after other contaminating proteins have been washed away, reduced glutathione is added to elute the GST-fusion protein from the resin (Figure 5)

Proteins were analyzed by immunoblotting using the following primary antibodies: anti-Gstt1 (Abcam, ab175418), -actin (Sigma-Aldrich, A5316), Tubulin (Sigma-Aldrich, T6199), E-Cadherin (BD, 610181), Vimentin (BD, 550513), PCNA (PC10) (Santa Cruz, sc-56), Fibronectin (Abcam, ab199056), GSH (D8) (Santa Cruz, 52399)