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Certain antioxidant compounds, such as ascorbic acid and dehydroascorbic acid, can directly react with 4,5-diaminofluorescein, forming fluorescent products with emission spectra similar to the green-fluorescent triazolofluorescein product (Magalhaes et al
For adolescents or adults, similar pathological changes are often caused by hypertension or aneurysm rupture, termed ischemic stroke
Nature 468:790797, 2010
