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cerevisiae BY4741 to replace the GSH1 promoter with the strong constitutive promoter pTEF1 by homologous recombination, with pgsh1 D:: HIS3MX6-pTEF1 transformants selected by growth in YNB minus histidine
Alternative upstream causes may include the inhibition of cofactor incorporation, or cofactor displacement, by certain toxins or heavy metals
