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coli AGE assay ELISA was used to detect carboxymethyl-lysine levels as in Pepper et al 26 with 100 l of supersensitive, 3,3,5,5-tetramethylbenzidine (TMB) (Sigma) used as the color reagent and read at 630 nm on a BioTek ELx808 absorbance reader
Efforts to restore normal trans-sulfuration metabolism in autistic individuals have shown varying degrees of success
Addressing relationship factors alongside peptide use optimizes outcomes
However, realistic expectations remain essential for long-term satisfaction
