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Sections were permeabilized using PBS-Triton, and blocking was carried out using 5% blocking buffer: 5% BSA, 1% goat serum (Jackson Immuno Research), 1% donkey serum (Jackson Immuno Research) in PBS-T
The inter hydrogen bonding between the drug and carrier(s) molecules makes the peak broaden and shifting of peaks to a higher frequency range
The lectures are video recordings from classes taught at Yale, Stanford University, Khan Academy, etc
pmid:10070083 6
