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FIGURE 2 The GF protocol produced HLCs with a mature differentiated morphology

Immunoblotting after SDS-PAGE of chemically lysed yeast 30 was performed using standard protocols with antibodies specific for yeast glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH, ThermoFisher, MA5-15738, 1:10.000) and HA (Sigma, H9658, 1:10000)

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