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In practice, this likely contributed a small amount of oxygen (from the wet paper towel), as EcN did typically form a biofilm ring at the air-liquid interface under this condition, which was absent for larger-volume biofilm cultures in culture tubes that were incubated strictly anaerobically without humidification

, 155 , 536-542, Stevens, G

doi: 10.3389/fendo.2023.1126661 42 StockwellBR

In addition to being able to hydrolyze asparagine to aspartate, the ASRGL1 encoded enzyme hydrolyzes isoaspartyl peptide linkages
