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Imaging was performed on an EVOS Digital Inverted Fluorescence Microscope (Invitrogen), using the FITC (green) and the DAPI (blue) filters

Validation of Compendial Procedures (HPLC method validation principles)

We focused on a peptide therapy in cytoprotection, using a small, orally active, anti-ulcer peptide (6, 9-11), stable in human gastric juice (12) with significant cytoprotective activity (6, 10, 11) - stable gastric pentadecapeptide BPC 157 (MW 1419), with very safe profile (LD1 could be not achieved, no side effects in clinical trials (13, 14), effective in trials for inflammatory bowel disease therapy (13, 14) and wound healing (15-18)

To assess treatment response, the study uses advanced imaging tools including intestinal ultrasound (IUS) and magnetic resonance enterography (MRE)