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Glutathione is a tri-peptide (L-g-glutamyl-L-cysteinyl-glycine) that acts as both reducing agent and capping agent, which assemble on the surface of AuNCs via a stable gold-thiol linkage (from cysteine) and imparts the nanoclusters with a net negative surface charge (zeta potential = 32 4.2 mV, pH = 7.5) due to the presence of terminal carboxylic acid functional groups (sketch depicting the assembly of glutathione molecules on GSH-AuNCs and the chemical structure of glutathione peptide is shown in Fig

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