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The RT-PCR mixture contained 5 L 5xQIAGEN OneStep RT-PCR Buffer, 1 L dNTP Mix, 1.5 L of 10 M sense primer, 1.5 L of 10 M antisense primer, 1 L OneStep RT-PCR Enzyme Mix, RNA as described and RNase-free water

Cyslt2 receptor-mediated aqp4 up-regulation is involved in ischemic-like injury through activation of erk and p38 mapk in rat astrocytes

Moreover, additional RSPO supplementation in LGR4 KO mice fails to rescue hepatocyte proliferation

Some reports have indicated that altered FSH-signaling and/or over expression of FSHR can actually promote apoptosis in unselected follicles, which may potentially happen via excessive accumulation of cAMP or activation of p38 MAPK pathways (106, 135)