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In addition, we studied NO/cGMP signaling and cytotoxicity in cultured endothelial cells and measured the NO-dependent and -independent activities of purified sGC in the presence of aerosol extracts

Materials and methods Materials Norharman-HCl, RPMI 1640 medium, fetal bovine serum (FBS), trypsin-EDTA, and penicillin-streptomycin were purchased from Sigma-Aldrich (Deisenhofen, Germany)

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Due to her passion, Abigail has gone on to earn two certifications including a certification in medical-surgical nursing (CMSRN) and a certification in holistic nursing (HNB-BC), in hopes of being able to better serve her patients