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Step 4: Clean the Stem Take a pipe cleaner and run it through the stem, ensuring that it reaches from the mouthpiece to the bottom where the stem meets the bowl

In this process, the histones that are characteristic of somatic cells are replaced by protamines in a stepwise process where transition nuclear proteins (TNP) are intermediary proteins

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Rev Environ Health 22:133149 Bencko V, Wagner V, Wagnerov M, Reichrtov E (1983) Immuno-biochemical findings in groups of individuals occupationally and non-occupationally exposed to emissions containing nickel and cobalt