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The genomic DNA was isolated from the spermatozoa using the QIAamp DNA Mini Kit, followed by mitochondrial DNA amplification via the REPLI-g Mitochondrial DNA Kit supplied by QIAGEN (Hilden, Germany), adhering to the kits instruction manual
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Sensitivity of mouse oocytes to nicotine-induced perturbations during oocyte meiotic maturation and aneuploidy in vivo and in vitro
The interior of the Astuccio pipe case is biscuit coloured soft suede and has two sewn in pipe loops and a matching leather pipe tool holder
