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Nuclear protein extraction HCE-T cells cultured in 60 mm dishes (Corning Inc.) were rinsed twice with ice-cold PBS and then harvested by scraping in the presence of 500 l of hypotonic buffer [20 mM TrisHCl (pH 7.4), 10 mM NaCl, and 3 mM MgCl 2 ] containing a protease inhibitor cocktail as well as phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich)

Doing so will be fundamental to levelling-up the health inequalities that have been so clearly exposed by the pandemic

Smokeless tobacco use has been shown to be a gateway drug not only leading to cigarette smoking, but the use of other drugs such as alcohol, marijuana, cocaine and inhalants

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