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Add a piece of bread or a half piece for small quantities

Chromatography was performed on an X-Bridge BEH C18 column (2.5 m particle size, 3 mm 150 mm, Waters, United States) at 50C with a flow rate of 0.6 ml/min, applying a gradient consisting of 20 mM ammonium formate in 10% methanol with pH 9 (A) and methanol (B)

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