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The timing and duration of receptor activation also influences outcomes
In a further set of experiments, IBMX (at a higher dose) was added only for the final 10 min out of the 16 h incubation for a point estimate of cAMP synthesis rate

Coronary effluents of isolated mouse hearts perfused with GLP-1(2836) showed a modest increase in extracellular cAMP levels that did not differ from levels in controls (Figure 2B)

Page MJ, Shamseer L, Tricco AC (2018) Registration of systematic reviews in PROSPERO: 30,000 records and counting
