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Description
Cells were washed with Hanks Balanced Salt Solution (Lonza) buffered with 20 mmol/l HEPES (Sigma) and supplemented with 0.1% BSA (Sigma) (wt/vol.)
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That 2.4% gap is due to metabolic resistance
(1999) Entry of CART into brain is rapid but not inhibited by excess CART or leptin
