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For calculation, 5-h fasting blood samples were collected from the tail, and glucose and insulin levels were determined using a digital glucometer (Roche) and a commercial ultrasensitive insulin ELISA kit (ALPCO

In additional analyses that used an alternative as-treated definition for drug use, the mean follow-up time among patients who started GLP1 receptor agonists was 1.8 years in the analysis with 90 day gap and tail periods, 2.6 years in the analysis with one year gap and tail periods, and 2.9 years in the analysis with two year gap and tail periods

Before the study began, all participants had magnetic resonance imaging (MRI) to evaluate brain structure and volumes, glucose metabolism with PET scans and other tests looking at memory, comprehension, language and spatial orientation

Given the relatively smaller reduction in body weight (9.4%) in the SELECT trial compared to previous RCTs: 14.9% in STEP1 (BMI 37.86.7 kg/m 2 ) [16] and 13.2% STEP6 (BMI 31.9 kg/m 2 ) [49], pleiotropic effects of semaglutide may contribute to the overall CV risk reduction [25,37]
