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[46] through a combination of coimmunoprecipitation, RNAi knockdown, immunoblotting, cAMP assays, and pharmacological assays demonstrated that -Arrestin-1 knockdown broadly attenuated GLP-1 signaling, causing decreased ERK and CREB activation and IRS-2 expression as well as reduced cAMP levels and impaired insulin secretion

conducted a randomized, double-blind trial, so neither participants nor researchers knew who was receiving the drug versus a placebo over 400 adults with both obesity (BMI 30) and knee osteoarthritis (radiographically confirmed and quite painful)

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