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Aulinger, B
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10.7326/0003-4819-143-8-200510180-00006 54 HeppnerK
Cells were harvested by centrifugation at 4,000 g and resuspended in lysis buffer (20 mM Tris, 300 mM NaCl, 5 mM imidazole, 0.7 % CHAPS, pH 8.0) supplemented with protease inhibitors (Thermo Fisher Scientific) and bovine pancreatic DNase I (Sigma-Aldrich)
