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Cortisol levels can be measured through various methods, including blood tests, salivary samples, and 24-hour urine collections

After washing with TBST, the immune complexes were detected via chemiluminescence (Beyotime, Shanghai, China) using a Pierce western blotting kit (Thermo Fisher Scientific)

Expectedly, the increase in PCWP during exercise was attenuated, but there was no change in peak VO 2

Monitor fiber, protein, calories, and water for better results
