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A 10 l qPCR reaction was amplified in duplicate wells for each sample 4.5 l of cDNA mixture (100 ng cDNA template + rest nuclease-free water) with 5 l iTaq Universal SYBR Green Supermix, and 0.5 l forward and reverse primer mixtures (10 pm)

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Rapid or excessive weight loss can increase the risk of gallstones, loss of muscle mass and bone density, and nutritional deficiencies. Some people, including older adults, postmenopausal women, and individuals whose weight loss approaches a range considered underweight may be at particularly increased risk for negative health impacts from rapid or excessive weight loss, Moiz says