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Consistently, radioligand competition studies combining isolated C-terminal (ECD) and N-terminal (7TMD) GLP-1R constructs with native and N-terminally or C-terminally truncated GLP-1 and exendin-4 showed that: 1) GLP-1 binding is primarily determined by interactions with the ECD, but also requires interactions with the 7TMD of GLP-1R

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