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Materials and methods HepG2 culture We obtained the human hepatoma HepG2 cell line (HB-8065, ATCC) from ATCC (Manassa, Virginia, USA) and maintained it in Dulbecco's modified Eagle's medium (DMEM) (31966047, Gibco, Massachusetts, USA) at 37 C and 5% CO2

Comparative studies and head-to-head trials are essential for understanding the distinct attributes of each GLP-1 RA, shedding light on their efficacy, dosing, and side effects to better tailor treatment plans to individual patient needs

Clinical trials of GLP-1 receptor agonists in MASH have noted reduced levels of liver enzymes, markers of liver injury, and improved histologic markers of inflammation, although the precise anti-inflammatory mechanisms independent of concurrent improvements in metabolic parameters remain unclear [27, 30]