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Immunofluorescence was performed using the following primary antibodies: CD3 (Abcam), CD8 (Abcam), E-cadherin (Invitrogen), Pan-citokeratin (Invitrogen), alpha-SMA (Invitrogen), P4HA1 (Abcam), and Hydroxyproline (Cell signaling)

Except for patients aged 60 years or above, the improvement in glycemic control due to 5:2 meal replacement was observed across all participants, including obese or overweight patients

Not after the weight stabilizes
