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Using single neuron ex vivo electrophysiological recordings from genetically identified histaminergic (HDC) neurons, we demonstrated that approximately 40% of histaminergic neurons are excited by the non-selective MC3R/MC4R agonist melanotan II (MTII) or a selective MC4R agonist (THIQ) (147)
Calcium assay in CHO-GLP-1R cells PathHunter GLP-1R cells were loaded with calcium dye (Calcium 6, Molecular Devices) in HBSS plus 20 mM HEPES and 2.5 mM probenecid 1 M agonist for 90 min at 37 C
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