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Description
Maintain aseptic technique during all handling and reconstitution steps
Between-study heterogeneity was negligible (I = 0%), supporting a robust and reproducible pattern of gastrointestinal disturbance
For islet perifusion, 75 islets were handpicked and loaded into 0.275 mL chambers containing KRPH (140 mM NaCl, 4.7 mM KCl, 1.5 mM CaCl 2 , 1 mM NaH 2 PO 4 , 1 mM MgSO 4 , 5 mM HEPES, 2 mM NaHCO 3 , 1% fatty acid-free BSA) in 2.7 mM glucose
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