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A., Lombardo, F., Dominy, B

There is no evidence that retatrutide directly causes hair loss

To construct the GPCR expression vectors, we utilized the Gateway system and subcloned 258 full-length human GPCR cDNAs from pDONR201 vectors 51 into pcDNA3.2/V5-DEST vectors (Thermo Fisher Scientific)

Then, all animals were euthanized by bleeding after transection of the abdominal vena cava under anesthesia and the liver, heart, soleus muscle, gastrocnemius muscle, tibialis anterior muscle, extensor digitorum longus muscle, epididymal white adipose tissue, and brown adipose tissue were harvested and immersed in RNAlater solution (Qiagen, Venlo, Netherlands) for qPCR analysis