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And definitely dont add any glutathione to your binding buffer save that for the elution step

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Its hard to make sense of which NAD supplement will be best for you, so your best bet might be to get adequate niacin in your diet. Some foods like refined grains, refined fats, processed foods and sugar might deplete your niacin status, so balance those out with the foods highest in niacin: liver, fish, poultry, other meat, and peanut butter