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These cell lines were cultured in DMEM/Hams F-12 (1:1) (Thermo Fisher Scientific, 12634010) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, A5256701) and 1% penicillin and streptomycin (Thermo Fisher Scientific, 15140122) at 37 C with 95% humidity and 5% CO2

Namely, upon encountering the regular vicious course of double obstruction of vessels, as individual agents, both L-NAME and L-arginine affect the course of the lesion in a particular way, and neither appears to be completely effective

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