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The formation of ice crystals can physically damage the delicate protein structure of a peptide, a process called denaturation

Importantly, animals were singly housed to eliminate the exchange and cross-contamination of intestinal microbiomes, a factor that potentially biases studies with group-housed animals by minimizing microbiome variations for co-housed animals within a cohort

[PMC free article] [PubMed] [Google Scholar] 2.DeFoor MT, Dekker TJ: Injectable therapeutic peptidesAn adjunct to regenerative medicine and sports performance