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After 24 h of drug treatment or cystine starvation, 10 l of CCK-8 reagent was added to each well containing 100 l medium in total and the plate was incubated at 37 C, 5% CO 2 for 3 h
Subsequently, expression began to decline at 36 hours post-inoculation ( Figure 3 )
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melanogaster, constitutive ubiquitous overexpression of CBS extended a lifespan, improved locomotor activity, and increased resistance to hyperthermia (35 C) [166]
