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RES) in mammalian brain and in vitro [53]

In CNS- Glud1 +/ /Stress mice, stress may limit the ability of the mPFC to regulate glutamate homeostasis [64, 65], and hinder the competency of the mPFC to implement compensatory mechanisms critical for intact homeostasis at the glutamate tripartite and glutamate-GABA synapses

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Therefore, a 1.1 kb fragment containing gliT was amplified using ogliT- Bgl II and ogliT- Not I and subcloned into pCR2.1-TOPO (Invitrogen)