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After centrifugation at 65,000 g for 30 min, the supernatant was collected and incubated with 10 ml Ni-NTA resin (Cytiva) in the presence of 8 mM imidazole to prevent the nonspecific binding at 4 C overnight

Sustained lipid peroxidation leads to the accumulation of harmful molecules such as lipid peroxides, aldehydes, and their conjugates, ultimately resulting in rupture of the plasma membrane [105,106]

This was associated with rapid proliferation due to metabolic adaptation and improved fitness, resulting in high fungal burden in vital organs 10

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