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Option 2: Full reconstitution with portion freezing Reconstitute the entire 100mg vial, then immediately divide into multiple sterile vials

every 4 days, total 4 times or more

We simulated the effect of pifithrin- treatment by multiplying the phospho-p53-dependent production of p21 and MDM2 with k PFT = 0.5, and the amount of phospho-p53 and the phosphorylation rate of p53 with k PFT phos = 0.67 at the time of treatment, because the effect of pifithrin- causes approximately a 50% decrease in p53-dependent transcriptional activation 95 and a 33% decrease in phosphorylated p53 species 93

Cell 187 , 19551970.e23 (2024)