kinetic study on the reactions of platinum drugs with glutathione Schematic representation A: Mode of action of platinum-based drug Oxidative Stress Induced by Pt(IV)
Description
Efficiency in the glutathione regeneration process is crucial to keeping cells in a healthy, reduced state
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T.YaoY.HuangX.et al (2023)
DNA templates for in vitro transcription of sgRNA were generated by annealing a scaffold oligonucleotide sequence: AAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC, with gene-specific oligonucleotides containing the SP6 promoter sequence (GCGATTTAGGTGACACTATA), a 20-base target sequence (see Table S1), and a complementary sequence to the scaffold oligo (GTTTTAGAGCTAGAAATAG)
