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Description
Inhibiting glycolysis rescues memory impairment in an intellectual disability Gdi1-null mouse
Ribeiro CT, Gasparotto J, Petiz LL, Brum PO, Peixoto DO, Kunzler A, da Rosa Silva HT, Bortolin RC, Almeida RF, Quintans-Junior LJ, Arajo AA
Extracted brains were post-fixed overnight in the same fixative at 4 C, then cryoprotected in 30% sucrose solution for 48 h
Two-step quantitative reverse transcription PCR (qRT-PCR) Total RNA from cells was extracted using TRIzol (Thermo Fisher, 15596018) according to the suppliers protocol and any contaminating DNA removed with TURBO DNA- free Kit (Thermo Fisher, AM1907)
