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ROS are unstable oxygen-containing molecules that easily react with other molecules in a cell [14] and are generated after physiological dysfunctions or exposure to physical agents, such as ultraviolet rays and heat, chemical factors, environmental toxicants, smoking, alcohol, or even other factors, such as obesity, varicocele, aging, or diabetes [11]

Due to high concentration of L-carnitine in epididymis and its potential in stabilizing sperm plasma membrane and increasing sperm survival, and reduction of sperm intracellular L-carnitine level following cryopreservation, (77) some studies have suggested that adding L-carnitine to cryomedium can compensate its loss in sperm cell and improve sperm parameters during freeze-thaw process

Stereology as a valuable tool in the toolbox of testicular research

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