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The semen was sent to the laboratory immediately after collection, and the sperm sample was evaluated for sperm density and sperm motility at 3538C

After incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Thermo Fisher Scientific, 1:5000), the proteins were visualized using chemiluminescence reagents (29050, Engreen, San Jose, CA, USA) with a Gel Doc EZ Imager (Bio-Rad Laboratories, Hercules, CA, USA) and analyzed using the Image Lab software (Bio-Rad Laboratories)

While some studies report improved sleep quality, others have found reductions in sleep duration (Kerkeni et al., 2024)

PMID: 35343261